Lipid profiling analyses from mouse models and human infants.
STAR protocols 2022 ; 3: 101679.
PubMed ID : 36115026
PMCID : PMC9486117
This protocol outlines a translational lipidomic approach to discover lipid biomarkers that could predict morphometric body and histological organ measurements (e.g., weight and adiposity gains) during specific stages of life (e.g., early life). We describe procedures ranging from animal experimentation and histological analyses to downstream analytical steps through lipid profiling, both in mice and humans. This protocol represents a reliable and versatile approach to translate and validate candidate lipid biomarkers from animal models to a human cohort. For complete details on the use and execution of this protocol, please refer to Olga et al. (2021).
Lipidomics is a technique to comprehensively analyse all different molecules in a biological sample. This tool that can be used in translational research to validate mechanisms, observed in model system, to humans. Lipidomics in different mouse models showed that specific enzymes in fatty acid metabolism in early life, named desaturases, are linked to changes in later life body composition. We showed that these desaturase enzymes in humans at 3 months of age, are also linked to childhood obesity risk. In this paper, we describe the methods in full detail to enable other researchers to use or repeat this work.